Category: Science

calcium assay

The Fluorescence-Based Calcium Assay &the GCaMP Family of GECIs

This article discusses the Fluorescence-based calcium assay and the GCaMP family of GECIs. It also covers the PBX Signal Enhancer. The Fluorescence-based calcium assay requires the removal of media prior to detection. It is a rapid method for calcium determination and is compatible with a wide range of cell types. You can also use it in conjunction with other calcium assays to assess the concentration of various calcium ions in aqueous solutions.

GCaMP family of GECIs

GECIs are fluorescent compounds engineered to change their fluorescence based on the presence of a single fluorophore. The GCaMP family of GECIs consists of a circularly permuted form of the green fluorescent protein (GFP) linked to calmodulin (CaM) at either its N-terminus or C-terminus. …